Modifying Enzymes
Thermo Scientific™ T4 RNA Ligase (10 U/μL)
Catalyze the ATP-dependent formation of phosphodiester bonds between 5'-P and 3'-OH termini of oligonucleotides, single-stranded RNA and DNA.
Thermo Scientific™ TheraPure™ Modifying Enzymes
Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.
Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 20 U/μL
Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA
Invitrogen™ Bacterial Alkaline Phosphatase
Removes 3' and 5' phosphates from DNA and RNA
Thermo Scientific™ Exonuclease III (200 U/μL)
Digest double-stranded DNA or DNA-RNA hybrids with Exonuclease III is a 3'–>5' exonuclease.
Invitrogen™ Gateway™ BP Clonase™ II Enzyme mix
Catalyzes in vitro recombination of PCR products to generate entry clones
Invitrogen™ LR Clonase II for BaculoDirect™ Kits
Eukaryotic system for high-level protein expression in insect cells
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
Thermo Scientific™ Uracil-DNA Glycosylase (1 U/μL)
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.
Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Invitrogen™ Terminal Deoxynucleotidyl Transferase (TdT) Buffer
Supplied with Terminal Deoxynucleotidyl Transferase (TdT)
Invitrogen™ S1 Nuclease
Single-strand-specific endonuclease hydrolyzes single-stranded RNA or DNA into 5' mononucleotides